TABLE 4.
Detection of anthrax PA in mice injected with different doses of PA and LF by ENIAa
| Toxin dose injected (μg)
|
No. of animals | PA concn measured in blood (μg/ml) | |
|---|---|---|---|
| PA | LF | ||
| 50 | 50 | 2 | 1.37 ± 0.42b |
| 50 | 25 | 2 | 1.41 ± 0.09 |
| 50 | 5 | 2 | 1.11 ± 0.21 |
| 10 | 50 | 4 | 0.83 ± 0.36 |
| 10 | 25 | 4 | 0.81 ± 0.28 |
| 10 | 5 | 2 | 1.09 ± 0.05 |
| 10 | 0 | 2 | 0.14 ± 0.04 |
| 0 | 50 | 2 | 0.00 |
| 0 | 25 | 2 | 0.00 |
| 0 | 5 | 3 | 0.00 |
a
BALB/cJ mice (n = 2 to 4) were injected with different amounts of wild-type PA or LF or with premixed combinations of these toxins (all prepared in PBS) and were bled 2 h after injection. PA levels in plasma were assessed by ENIA. The samples were diluted from 1:10 to 1:1,000.
b
The data are means ± SDs.