Figure 2.
All subunits of TORC2 localize to the plasma membrane in a similar pattern. (a) All exclusive TORC2 subunits show a similar pattern at the plasma membrane. Strains carrying the indicated subunits of TORC2 as the only allele of the corresponding gene were analyzed by confocal microscopy. Representative midsections (left) and top sections (right) are shown. White lines delineate the perimeter of the cell. Bar, 5 μm. (b) Each TORC2 focus contains two to six copies of each subunit. GFP signals measured from unprocessed cell surface images were quantitated and related to a GFP standard. The median of the signal from >100 measurements is shown as a red diamond, the mean is shown as a white line in a gray box, outlines of the box indicate the 0.25 and the 0.75 quartiles. Error bars are 1.5 interquartile ranges from the mean. For details, see Supplemental Figure 1 and Materials and Methods.