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The hairpin fluorescence probe and oligonucleotides
| Name | Sequence (5′ to 3′) |
|---|---|
| Hairpin fluorescence probe | FAM-TCTTGGACACA GTAAAGAGAGGTGCGCCCAT TGTGTCCAAGA-DABCYL |
| Target | ATGGGCGCACCTCTCTTTACTGTGTC TTT |
| Random DNA | TGCAAGGTGTCAGTATAATCCGACGT TTT |
| Primer | TCTTGGAC |
The italicized region of the hairpin fluorescence probe identifies the stem sequence and the underlined region identifies the complementary sequence to the primer.
