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The hairpin fluorescence probe response to the target. Experiments were performed in the absence of polymerase and dNTPs with 5 × 10–8 M probe and different concentrations of target. The curves from a to e contain the target with 1.25 × 10–8, 2.5 × 10–9, 5 × 10–10, 1 × 10–10 and 0 M, respectively. All samples were incubated at 37°C.
