Figure 8.
Constitutively active MEK is sufficient to induce HIF-1 activity in GCs. GCs were transfected with HRE-(3)-TK-Luc construct (A) or VEGF-Luc (B), as described in Materials and Methods and with 50 ng of pCDNA3 or R4F-MEK, as indicated. GCs were left untreated (CON) or treated with 50 ng/ml IGF-I, 50 ng/ml FSH, or 50 ng/ml IGF-I + 50 ng/ml FSH, as indicated, for 6 h. Values are expressed as a mean ± sem of triplicates and representative of three separate experiments. Student’s t test for compared values: **, significant difference with P ≤ 0.05 between lanes 1 and 2, 1 and 3, 2 and 4, and 1 and 6 in A and between lanes 1 and 2 and 5 and 6 in B. Lanes 2 and 6 in B are not significantly different.