FIGURE 7.
BM-derived cell expression of C5aR is required for optimal T cell IFN-γ production in vivo. A, Representative flow cytometry plots of CFSE-labeled CD4+ Mar T cells of Mar T cells injected into WT (Thy1.1)→C5aR−/− (Thy1.2) or C5aR−/− (Thy1.2)→WT (Thy1.1) BM chimeric mice. Chimerism was determined to be >90% donor BM based on Thy1.1/Thy1.2 polymorphisms (inset). B, Percentages of injected IFN-γ+ Mar T cells 72 h after injection into WT→WT, C5aR−/−→ C5aR−/−, WT→C5aR−/−, or C5aR−/−→ WT BM chimeric mice. Data represent mean values for groups plus SD; n = 3/group. *, p < 0.05 vs WT→WT.