FIG. 1.
Recombinant RV genomes. The genome of wt RV SAD L16 comprises five cistrons defined by transcription stop/restart signals (arrowheads) that give rise to five capped mRNAs as indicated. The IRES reporter virus SAD RL-IRES-FL contains an extra gene from which a bicistronic mRNA encoding RL and FL is transcribed. In SAD HRV2-P and SAD PV-P, here represented by the genome of SAD IRES-P, the transcription signal sequences of the N/P gene border are replaced by the IRES elements of HRV2 and PV, such that P protein expression depends on internal translation initiation by the IRES elements.