Table 1.

An approximate accounting of the impact of lethal cell injury of HL-60 cells, which leads to immediate necrosis or delayed apoptosis, on the results for FD500 cell loading or gene transfer and expression. The non-necrotic percentage (NNP) is listed from the trypan blue counts soon after treatment. The flow cytometry results for cell loading and gene expression in the non-necrotic cells at 6 hr post exposure (I. e. from Fig. 5) are listed as the flow cytometry positive percentage (FCP). This FCP, reduced by the NNP (I. e., FCP × NNP/100), is listed as the necrosis-corrected percentage. However, many of the FCP cells were apoptotic (see Fig. 5), and a further adjustment for the non-apoptotic percentage is listed as the apoptosis adjusted percentage, which reflects the actual positive percentage in surviving cells from the entire pre-exposure suspension. Statistical P values are for the final apoptosis adjusted result relative to the sham result. After accounting for the lethal cell injury, the sonoporation effects peaked at 0.4 MPa and declined at 0.6 Mpa.