Figure 3. HIF-1α and VEGF Expression.
Immunohistochemistry and Western blots for HIF-1α and VEGF expression after hypoxia-ischemia injury. (A) Immunostaining for HIF-1α and VEGF in the cortex of the ipsilateral hemisphere at 24 h after HI. Compared with the HI group, HIF-1α and VEGF expression was reduced in the 2ME2 group. Arrowheads indicate cells that are positive for HIF-1α and VEGF. (B and C) Representative Western blot analysis showed that HIF-1α and VEGF (with β-actin as a loading control) were expressed in the ipsilateral hemisphere at 24 h after HI respectively. Quantification of the Western blot analysis showed increased HIF-1α and VEGF in the HI group and DMOG-treatment group compared with both the sham group and the 2ME2-treatment group (*P < 0.001, versus sham; #P < 0.05, versus HI; vertical bars indicate SEM).