Figure 3.
Expression of Cdkn1a mRNA and protein upon introduction of ESCC miRNAs. (a) Quantatitive PCR. mRNA expression was normalized to mock transfected Dgcr8 Δ/Δ ES cells. Error bars indicate s.d except for miR-1, for which the error bar indicates range. n=2 for miR-1. n=3 for wild type and n=4 for ESCC miRNAs. (b) Representative Western blot of Cdkn1a protein. (c) Densitometry for Western analysis of Cdkn1a protein. Data was normalized to mock transfected Dgcr8 Δ/Δ ES cells. Numbers of independent transfection experiments performed are indicated on the top of each bar. Error bars indicate the range.