TABLE 2.
Mitochondrial content in lentiviral transductants
| Lentiviral transductant | MitoTracker Green FM fluorescencea(mean ± S.E.) | Mitochondrial contentb(% cytoplasmic area ± S.E.) |
|---|---|---|
| % | ||
| Control shRNA | 647 ± 125 (1.0)c | 16 ± 1 (1.0) |
| PRC shRNA1 | 1360 ± 84 (2.1) | 34 ± 1 (2.1) |
| PRC shRNA4 | 855 ± 58 (1.3) | 19 ± 2 (1.3) |
a
Mean fluorescence intensity of MitoTracker Green FM as quantifies by flow cytometry is the average of at least three separate determinations ± S.E.
b
Mitochondrial content expressed as a percentage of cytoplasmic area ± S.E. is derived from morphometrical analysis of 10 digital electron micrographs for each transductant, such as those shown in Fig. 8
c
The numbers in parentheses indicate the -fold differences in mitochondrial content relative to the control