FIGURE 1.
Silencing of PRC expression in U2OS cells by lentivirally delivered short hairpin RNAs. A, total cell extracts were prepared from wild type U2OS cells (wt) and from stable lentiviral transductants expressing the control shRNA, lamin shRNA, PRC shRNA1, or PRC shRNA4. PRC and lamin A/C proteins were detected by immunoblotting following denaturing gel electrophoresis using rabbit anti-PRC-(1047–1379) and rabbit anti-lamin A/C antibodies, respectively, following division of the membrane at the indicated position (dashed line). Molecular mass standards in kilodaltons are indicated at the left. B, total cell extract was prepared from wild type U2OS cells, adenovirus-infected U2OS cells expressing either GFP (Ad-GFP) or PGC-1α (Ad-PGC-1α), and U2OS cell lentiviral transductants expressing control shRNA, PRC shRNA1, or PRC shRNA4. PGC-1α protein was detected by immunoblotting following denaturing gel electrophoresis using rabbit anti-PGC-1α serum. The arrows indicate the positions of PRC, PGC-1α, and lamin A/C.