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. Author manuscript; available in PMC: 2009 Jan 20.
Published in final edited form as: Nat Genet. 2007 Dec 9;40(1):43–50. doi: 10.1038/ng.2007.30

Figure 2.

Figure 2

Myc associates with repressed pri-miRNA promoters. (a) Representations of repressed pri-miRNAs of known structure. (b) Real-time PCR amplicons for ChIP were designed within 250-bp windows immediately upstream of the transcription start site (amplicon S), 500-bp windows upstream of amplicon S (amplicon U) or 500-bp windows downstream of amplicon S (amplicon D). (c) Real-time PCR analysis of Myc chromatin immunoprecipitates. Fold enrichment in this and subsequent ChIP experiments represents the signal obtained after Myc immunoprecipitation relative to the signal obtained after immunoprecipitation with an irrelevant antibody. A validated Myc-bound amplicon in the promoter region of CDKN1A was used as a positive control. The 50-fold enrichment threshold for positive Myc binding is indicated as a broken line. Data are mean ± s.d. from three independent measurements.