TABLE 1.
TLR2 KO Mice Develop a Neutrophilic Alveolitis following S. rectivirgula Exposure
| Alveolitis (cells×106)a |
Neutrophils (cells×106)b |
|||
|---|---|---|---|---|
| WT | TLR2 KO | WT | TLR2 KO | |
| Saline exposure | 0.21 ± 0.10 | N.D. | 0.001 ± 0.0009 | N.D. |
| S. rectivirgula 6 h exposure | 11.1 ± 1.5 | 9.5 ± 3 | 11.0 ± 1.4 | 9.0 ± 0.3 |
| S. rectivirgula 24 h exposure | 2.9 ± 2.6 | 1.2 ± 0.726 | 1.5 ± 1.0 | 0.7 ± 0.8 |
WT (n=9/group) or TLR2 KO (n=4/group) mice were exposed to saline or S. rectivirgula (30μg) one time and sacrificed at 6 or 24 h after exposure.
a
BAL was performed, and the recovered cells were counted using trypan blue dye exclusion. N.D., Not determined.
b
Total neutrophil cell count was derived by staining cells recovered from the BALF with antibody to CD45, Gr-1, CD11b, and F4/80; neutrophils were identified as Gr1+/CD45+/CD11b+/F480-. The cells were run on a BD LSRII flow cytometer, and the data were analyzed using FACSDiva software. There was no significant difference between WT and TLR2 KO mice in alveolitis or neutrophil cell count at any time-point tested.