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Serum leptin and insulin concentrations in 18-week-old fed or fasted male mice (n = 5). Blood was collected between 8 and 9 a.m. Mice were fasted for 48 h, which produced a fall in leptin and insulin in each genotype (✽, P < 0.05 [fed versus fasted] for both ghrelin+/+ and ghrelin−/− mice). There was no significant difference in the levels of leptin and insulin between each genotype whether they were fed or fasted (P > 0.05 [ghrelin+/+ versus ghrelin−/− mice]).
