Figure 2.
Examples of LSPS maps of excitatory synaptic inputs to YFP-positive and YFP-negative layer 5 neurons. (A) Depiction of recording arrangement, illustrating laser beam, mapping area (dashed boxed), and recording electrode. (B) A portion of a typical LSPS trace map, showing synaptic responses evoked at different sites around the neuron (Δ, soma). Gaps are where traces were excluded due to direct dendritic stimulation; such sites are rendered as black pixels in maps. Image on left shows bright-field appearance of slice. (C) Example of input maps for a pair of YFP-positive (left) and YFP-negative (right) neurons in upper layer 5, from the same slice. The dashed box indicates the pixels corresponding to the traces shown in (B). The color bar applies to both maps. (D) Another example; note predominance of layer 3 input to the YFP-positive neuron, and layer 2 input to the YFP-negative neuron. (E) Examples of maps for neurons in lower layer 5.