Table 3.
Purification of rPPK
| Fraction | Activity, units × 10−6 | Specific activity, units × 10−6/mg of protein |
|---|---|---|
| Lysate pellet | 15.2 | 1.0 |
| Solubilized membrane | 16.1 | 1.2 |
| Ni-agarose | 6.9 | 9.2 |
Purification of rPPK
| Fraction | Activity, units × 10−6 | Specific activity, units × 10−6/mg of protein |
|---|---|---|
| Lysate pellet | 15.2 | 1.0 |
| Solubilized membrane | 16.1 | 1.2 |
| Ni-agarose | 6.9 | 9.2 |