FIG. 2.
rAd41 vector induced cellular immune responses comparable to those of rAd5 vector with a single intramuscular immunization. Mice were immunized once with rAd5-Env or rAd41-Env by the intramuscular route and sacrificed 3 weeks later. (A) Representative flow plots of Dd/PA9 tetramer staining for each vector are shown. SSC, side-scatter characteristics. (B) The numbers of HIV Env-specific Dd/PA9+ CD8 cells were examined in PBMC by flow cytometry. (C) HIV gp140-specific IgG titers were measured in serum. Bars represent the mean values. *, P < 0.02; **, P < 0.01.