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. Author manuscript; available in PMC: 2008 Dec 29.
Published in final edited form as: J Neurochem. 2006 Jun;97(5):1467–1480. doi: 10.1111/j.1471-4159.2006.03841.x

Fig. 6.

Fig. 6

FACS-purified NPs can generate other neural subtypes. FACS-purified GFP+ cells were fully differentiated and analyzed by immunocytofluorescence using antibodies against serotonin (a–c), GABA (d–f) and glutamate (g–i). Shown are representative fields using confocal microscopy at 40× magnification. Scale bar = 50 μm. (j) sox1-GFP+ cells were fixed after neuronal differentiation stage (ND d10) and numbers of 5HT+, GABA+, glutamate+ and Hoechst+ cells were counted in multiple random fields per each sample. The proportion of each phenotype was calculated by dividing the cell numbers by the number of Hoechst+ cells. Each group represents an average of three samples from each independent experiment.