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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Matrix Biol. 2008 Mar 6;27(6):573–585. doi: 10.1016/j.matbio.2008.02.008

Figure 7. Fibroblast-derived 3D matrix responses to taxol are independent from FAK and Akt pathways.

Figure 7

Cells were cultured on control 2D plastic or onto fibroblast-derived 3D matrices in the presence or absence of taxol, β1-integrin functional blocking antibody mAb13, or a combination of both taxol and mAb13. A. Representative Western blot analyses of total FAK or Akt and activated pFAK-Y397 (pFAK) and pAkt-S473 (pAkt). GAPDH served as loading control. B, C. The ability of mAb13 to reverse the matrixinduced responses to taxol through FAK (B) or Akt (C) activities, were expressed as the log ratio ((plastic:matrix)taxol/mAb13) using the multivariate delta method, and the Wald test to assess statistical significance, in analysis of three independent Western blots of experiments performed in triplicate. In the graphs (B and C), point estimates and associated 95% confidence intervals constructed using the standard error of the log ratio estimate. Log ratio points with error bars that cross the line depicting a ratio that is equal to one are insignificant.