Figure 4.
Nur77-dependent expression of multiple glucose utilization genes. (a) Electroporation of Nur77-cDNA into denervated rat hind limbs rescues glucose metabolism gene expression. Both hind legs of each rat were denervated as described. 60 μg of Nur77 cDNA was injected and electroporated into EDL muscle in one leg, and pcDNA 3.1 empty vector (Ctrl) was injected as control in the other leg of the same animal. Animals were sacrificed after three days. We observed Nur77-dependent rescue in 2 of 4 animals. (b) Nur77 knockdown reduces the expression of glucose utilization genes. 100 μg of pSUPER vector encoding shRNA of Nur77 (shNur77) was electroporated into rat EDL muscle in one leg and scramble sequence (scr) was used in the other leg as control. Rats were sacrificed after 6 days. For both (a) and (b), total RNA was extracted for RT-PCR, and total crude membrane was extracted for western blot. GAPDH and Cav3 served as controls for each case.