. Author manuscript; available in PMC: 2008 Dec 9.

Published in final edited form as: Curr Genet. 2007 Apr 6;51(5):309–319. doi: 10.1007/s00294-007-0127-0

Table 3.

Maintenance of [PIN⁺] in rnq1-Δ pCUP:rnq1-x:GFP cytoductant

	rnq1 allele	Number of cytoductants obtained from the following donor

		[pin⁻]			High [PIN⁺]			Low [PIN⁺] ^a)
		Total	GFP foci	[PSI⁺] induction	Total	GFP foci	[PSI⁺] induction	Total	GFP foci
	no RNQ1	15	0	0	15	0	0	6	0
C	Δ4	9	1	1	12	1	1	9	0
	Δ5	16	0	0	14	2	2	9	0
	Δ6	15	0	0	14	7	7	16	2
	Δ7	14	0	0	15	8	10	15	6
	WT	14	1	1	14	12	11	16	10
N	ΔN2	10	0	0	11	6	3	7	5
	ΔN1	12	4	3	12	10	8	12	9

A cytoductant was considered to have GFP foci if more than 10% of the fluorescent cells had aggregates. Three samples of ~100 cells were assayed for each cytoductant. [PSI⁺] induction was scored as an increase of Ade⁺ in response to overexpression of SUP35 (Fig. 5). “N” and “C” indicate N- and C-terminal deletions, respectively.

^a)

Transmission of low [PIN⁺] could not be scored by [PSI⁺] induction in the recipient strain background