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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Exp Neurol. 2008 May 29;213(1):114–121. doi: 10.1016/j.expneurol.2008.05.014

Figure 2.

Figure 2

Expression of TLR2 and TLR4 increase in cortical neuronal cultures in response to exposure to HNE. A. The levels of TLR 2, 3 and 4 mRNAs in neurons treated with HNE (10 μM). B and C. Immunoblots showing protein levels of TLR2 (95 KDa) and TLR4 (90 KDa) in cultured cortical neuronal cells that had been exposed to the indicated concentrations of HNE for the indicated time periods. Levels of both TLR2 and TLR4 were increased within 3 hours and remained elevated through 20 hours of exposure to HNE. Analyses were performed on pooled samples from 4-8 cultures.