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Experimental design. Human CD34+ cells were transduced by a lentiviral vector containing MGMT(P140K) as transgene after a 1-day stimulation with SCF, Flt-3L, and TPO. Following a 1-day transduction, cells were infused into NOD/SCID mice that had been given a conditioning dose of 30 mg/kg BG and 10 mg/kg BCNU the day before transplant. Mice were subsequently treated zero, one, or two times with 30 mg/kg BG and 7.5 mg/kg BCNU before sacrifice and analysis at 8–10 weeks after transplant.
