FIGURE 6.
The C-terminal PH domain drives FGD2 co-localization with cortactin in membrane ruffles. HeLa cells expressing either EGFP alone (top panel), EGFP-FGD2 fusion protein (second panel), EGFP-FGD2FYVEKT (third panel), or EGFP-FGD2ΔPH2 (fourth panel) were grown on coverslips prior to fixation and permeabilization with Triton X-100. The samples were stained for cortactin, a protein localizing to newly polymerized actin filaments, using a monoclonal antibody, followed by Alexa 586-goat anti-mouse IgG (red). Cells expressing EGFP-FGD2ΔPH2 were also stained for the endosomal marker EEA1 (bottom).