Figure 3.
Analysis of in vivo episomal expression from lentiviral vectors with short or large deletions in the U3 sequence. (a) Tissue compartment-specific GFP expression mediated by short-deletion or long-deletion vectors, with (top) or without (bottom) functional integrase, in the striatum and corpus callosum of the rat brain, measured three months after vector infusion. (b) Cell type-specific GFP expression mediated by short-deletion (lower) and long-deletion (upper) nonintegrating vectors in neural (fluorescing with NeuN, left side) and glial (fluorescing with GFAP, right side) cells of the rat brain, measured three months after vector infusion. A 20-micron size bar appears in the 113/IN- GFAP/GFP merge panel. (c) Long-term luciferase expression produced by short-deletion or long-deletion vectors, with or without functional integrase, in the mouse liver. Animals were imaged 10 days, 45 days, and 6 months after intraperitoneal injection of vector. (d) Quantification of luciferase expression described in (c). Each bar represents the average of luciferase measurements from three mice.