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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: J Invest Dermatol. 2004 Feb;122(2):337–341. doi: 10.1046/j.0022-202X.2004.22243.x

Figure 1. (A) PCR–single strand conformation polymorphism.

Figure 1

Analysis of BRAF exon 15. Amplified exon 15 fragments containing the normal (V599) and mutant (E599) variants exhibit differential mobilities, as indicated by the upper and lower arrows, respectively. The asterisk marks the presence of the mutant allele. Note that the cell line in lane 3 is homozygous for the E599 mutation (lane 1: Mel-Swift; lane 2: MGH-BO-1; lane 3: SK-Mel 28; lane 4: WM455; lane 5: SK-Mel 63). (B) BRAF genotypes showing loss of normal allele at codon 599.