Figure 7.
Effect of mutein in the regulation of IL-13–induced BAL inflammation and mucus elaboration. Mutein (500 μg/mouse/d, intraperitoneally) or vehicle (PEG) were daily administrated to the inducible IL-13 transgenic mice (CC10-rtTA-IL-13) from a day before transgene induction by Doxycycline (Dox). After 2 weeks of Dox induction, mice were killed and (A) differential count on BAL cells, (B) airway mucus evaluation by D-PAS stains (×20 original magnification), and (C) Muc5ac protein expression quantitated by densitometry after slot and immunoblotting with Muc5ac antibody were illustrated. Values in A and C represent the mean ± SEM of evaluations in a minimum of five animals. B is a representative of a minimum of three similar evaluations (*P < 0.05).