Figure 6.
Nse4 is constitutively associated with tDNAs. (A) Two examples of tDNA sequences bound by Nse4–3HA on Chr2 left arm. ChIP-on-chip results are enlarged to show Nse4 association with tDNA loci. Arrows below indicate the position of each tDNA. Distance from the left chromosome end is indicated in kilobases (kb). (B) All the genomic tDNAs are bound by Nse4. Nse4 ChIP-on-chip MAT score at each known tDNA locus was quantified and represented as a percentage of tDNAs bound by Nse4 for each MAT score category. Darker areas represent higher MAT scores, hence higher confidence for Nse4 enrichment. (C) Centromeric tDNAs preferentially recruit Nse4. All tDNAs localized within the centromeric and pericentromeric regions of each chromosome were quantified as in (B).