Fig. 4.
The canonical Wnt pathway does not enhance agrin-mediated clustering. Myotubes were treated with Dkk1 or Sfrp1 in the absence or presence of agrin and Wnt3. (A) Neither Dkk1 nor Sfrp1 significantly reduces the number of AChR clusters induced by agrin. (B) Dkk1 does not affect the clustering induced by Wnt3 and agrin together. However, Sfrp1 reduces the clustering effect of Wnt3 plus agrin to the level of agrin alone (Lower, ***, P < 0.001). (C and D) The Gsk3 inhibitor BIO significantly reduces the clustering induced by agrin (***, P < 0.001) or both agrin and Wnt3 (***, P < 0.001). (Scale bar, 20 μm.)