Fig. 2.
Propagation of AII-induced [Ca2+]c signals to the mitochondria in intact PGASMCs. Cells were loaded with both Mito Tracker Green (i images, shown in green) and rhod2 (ii images, shown in red). Following stimulation with 100 nM AII, there is a large increase in rhod2 fluorescence (bottom ii image, shown in red), which is colocalized with MitoTracker Green (overlaid image iii, shown in yellow). Notably, the images shown at the bottom were taken 1–2 min after the stimulation. By then, the [Ca2+]c signal had already decayed, thus above the nuclear region no visible increase of rhod2 fluorescence appears.