TABLE 4.
In vitro growth rates and virulence levels of V. vulnificus strains
| Strain | Genotype | Doubling time (min) in LB-N | Virulencea
|
||
|---|---|---|---|---|---|
| Log no. of CFU/g
|
% of mice with detectable infection | ||||
| Skin | Liver | ||||
| FLA602 | fadR::mini-Tn5Km2phoA | 29 | ND | ND | 0b |
| FLA1000 | ptsI::mini-Tn5Km2phoA | 25 | 8.4 ± 0.2 | 6.3 ± 0.3 | 100 |
| CMCP6 | Wild type | 18 | 8.5 ± 0.2 | 6.5 ± 1.5 | 100 |
For virulence, five iron dextran-treated mice were inoculated s.c. with approximately 1,000 CFU, and infection was measured between 14 and 22 h later. ND, not detected.
Compared with levels for FLA1000 and CMCP6 (P = 0.002; χ2 test).