TABLE 3.
Expression of putative FadR-regulated genes in V. vulnificus fadR::mini-Tn5Km2phoA mutant FLA602a
| Gene | Fold change (mean ± SD) in mutant | P |
|---|---|---|
| fabA | −8.4 ± 5.3 | 0.005 |
| fabB | −6.9 ± 1.5 | 0.0002 |
| fadD | −1.6 ± 2.1 | 0.1 |
| fadB | 4.2 ± 2.4 | 0.04 |
a
FLA399 and FLA602 were grown to exponential phase in LB-N, and RNA was extracted. qRT-PCR was used to measure changes with the 2−ΔΔCt method, using 16S rRNA as an endogenous control between fadR mutant FLA602 and wild-type FLA399. P values are for paired Student t tests comparing normalized threshold cycle values for the wild-type and mutant strains.