FIG. 5.
SEA suppresses TLR ligand-induced IL-12 production in MyD88−/− DCs. WT and MyD88−/− DCs were pulsed with the TLR3, TLR4, and TLR9 ligands poly(I:C), LPS, and CpG, respectively, for 24 h. IL-12p40 (A) and IL-12p70 (B) levels were measured by ELISA. SEA suppressed the MyD88-dependent IL-12p40 and IL-12p70 production induced by LPS and CpG. poly(I:C)- and LPS-induced TRIF-dependent IL-12p40, and IL-12p70 production was also suppressed in a MyD88-independent manner. Data represent the mean ± SE from duplicate wells. The experiment was performed three times with similar results.