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SEA augments CpG-stimulated IL-10 production independently of TLR4 and TLR2. WT, TLR4−/−, and TLR2−/− DCs were pulsed in vitro with the TLR9 ligand CpG, with or without SEA and the IL-10R-blocking Ab (αIL10R) for 24 h, and cytokine production was measured by ELISA. SEA augments the production of CpG-induced IL-10, which is more evident when endogenous consumption of IL-10 is blocked. Data points represent mean values ± SE from three experiments.
