FIG. 2.
SEA-mediated suppression of TLR-induced DC activation is TLR2 independent. (A) Immature DCs derived from B6 and TLR2−/− mice were pulsed in vitro with the TLR2 and TLR9 ligands P3C and CpG, respectively, either alone or in the presence of SEA (S/L and S/C, respectively, for LPS and CpG). MHC-II surface expression at 4 h poststimulation was measured by flow cytometry. Data are representative of three experiments. (B) LPS- and CpG-induced production of IL-12 in the presence or absence of SEA. Intracellular IL-12 at 6 h poststimulation was measured by flow cytometry. Data are representative of three experiments. (C) IL-12p40 production, measured by ELISA; DCs were pulsed for 24 h with TLR ligand LPS or CpG plus or minus SEA. Data points represent mean values ± SE from three experiments.