Figure 3.
MnSOD overexpression alters ATM phosphorylation. (A) Phosphorylated ATM levels (p-ATM): cells (1 × 105 per 60 mm dish) were irradiated with 6 Gy, harvested at 0.5 and 2 h post-irradiation and fixed in 70% ethanol. Ethanol-fixed cells were incubated with anti-phospho-ATM antibody (Ser 1981) followed by FITC-conjugated secondary antibody. The fluorescence of FITC stained cells was measured using flow cytometry. (B) Correlation plot of relative p-ATM levels (2 h) and relative MnSOD activity.