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In vitro evaluation of the AP overlapping vectors in HT1080 cells. 80% confluent cells were infected at an moi of 1000 vg particles/cell for each virus and AP expression was examined by cytochemical staining 48 h later. (A) Representative photomicrographs from the intact gene vector (AV.AP) infection or the overlapping vectors (AV.AP.Upstream and AV.AP.Downstream) co-infection, respectively. (B) Morphometric quantification of transduction efficiency. N = 5 for each group
