Figure 3.
Fml1 Limits Crossing Over during Mitotic DSB Repair
(A) Schematic showing the repair of a double-strand gap in ade6 on plasmid pAN1 by homologous recombination with ade6-M26 on chromosome 3. The M26 mutation is indicated by the filled circle. See Figure S3 for a full description of the assay system.
(B) Histogram showing the mean relative transformation efficiency (TE) of cut versus uncut plasmid in strains MCW1193, MCW2498, MCW2096, MCW1818, MCW3811, MCW2498, MCW2487, MCW2550, FO1192, and MCW2264.
(C) Histogram showing the percentage of Ade+ recombinants that are crossovers in the same strains as in (B). Error bars are the standard deviations about the mean.