Figure 1.
Treatment with histone deacetylase (HDAC) inhibitors preserves dopaminergic (DA) neuronal function in 1-methyl-4-phenylpyridinium (MPP+)-treated neuron-glia cultures. Midbrain neuron-glia cultures were treated with vehicle, sodium butyrate (SB) (a) or trichostatin A (TSA) (b) at the indicated concentrations in the presence (■) or absence (□) of 0.5 μM MPP+. MPP+ was added 30 min after SB and TSA pretreatment. DA neuronal function was evaluated by the [3H]dopamine uptake assay 7 d after the varying treatments. Results are expressed as percent of control and represent means ±S.E. of three separate experiments. * p<0.05, ** p<0.01, compared to untreated control groups; # p<0.05, ## p<0.01, compared to MPP+ alone group.