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RT-PCR analysis. RNA extracts from Vero cells treated for 6 h with or without 100 ng ml−1 SubAB, 100 ng ml−1 SubAA272B or 0.2 μg ml−1 thapsigargin were analysed by RT-PCR using primers (Table 1) specific for XBP1, BiP, CHOP, GRP94, ATF4 and GAPDH (internal control), as described in Experimental procedures. The expected mobilities of spliced and unspliced XBP1 amplicons are indicated.
