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. Author manuscript; available in PMC: 2009 Jul 8.
Published in final edited form as: Biochemistry. 2008 Jun 12;47(27):7295–7303. doi: 10.1021/bi800308q

Table 1.

Kinetic parameters for the reactions of 15-hLO-1 with arachidonic acids. Standard deviations from two independent experiments are indicated in parentheses.

KM (μM) kcat (s−1) Apparent Dkcat
15-hLO-1 and AA 3.7 (0.3) 9.93 (0.28)
15-hLO-1 and 13-d2-AA 2.5 (0.2) 2.49 (0.08) 3.99 (0.17)
15-hLO-1 and 10-d2-AA 3.4 (0.4) 9.26 (0.34) 1.07 (0.05)
15-hLO-1 and 10,13-d4-AA 2.8 (0.1) 1.00 (0.04) 9.93 (0.34)