Table 1.
Kinetic parameters for the reactions of 15-hLO-1 with arachidonic acids. Standard deviations from two independent experiments are indicated in parentheses.
| KM (μM) | kcat (s−1) | Apparent Dkcat | |
|---|---|---|---|
| 15-hLO-1 and AA | 3.7 (0.3) | 9.93 (0.28) | |
| 15-hLO-1 and 13-d2-AA | 2.5 (0.2) | 2.49 (0.08) | 3.99 (0.17) |
| 15-hLO-1 and 10-d2-AA | 3.4 (0.4) | 9.26 (0.34) | 1.07 (0.05) |
| 15-hLO-1 and 10,13-d4-AA | 2.8 (0.1) | 1.00 (0.04) | 9.93 (0.34) |