Figure 1.
Effect of MMPs expressed in COS-1 cells on random cell migration.
A, B) Expression of MMPs in COS-1 cells enhances cell migration. COS-1 cells transfected with corresponding cDNAs as indicated were examined by a Transwell chamber migration assay. Migrated cells were microscopically examined (A) and counted (B). *Significant difference; p < 0.05, as compared to COS-1 cells.
C, D) Comparable expression of MMPs in transfected COS-1 cells examined by Western blotting. COS-1 cells transfected with various cDNAs were lysed and immunoblot analysis was performed using the corresponding antibodies as indicated (C). Densitometric analyses of expression levels of MMPs and tubulin, respectively, in COS-1 cells were performed. Relative expression levels of MMPs in COS-1 cells were determined based on ratios between levels of specific MMPs and the corresponding respective levels of tubulin (D).