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. Author manuscript; available in PMC: 2008 Oct 27.
Published in final edited form as: Nat Neurosci. 2005 Dec 11;9(1):41–49. doi: 10.1038/nn1602

Figure 4. In vitro treatment with alkaline phosphatase shifts wild-type bslo channels into a state that is readily activatable by ethanol, but does not modify bslo T107V channel responses to alcohol.

Figure 4

(a) Before alkaline phosphatase (AP) treatment, acute exposure of the cytosolic side of I/O patches to 100 mM ethanol evoked varied responses in wild-type bslo: robust activation (filled circles) and refractoriness/inhibition (hollow circles). In contrast, after AP application (33 IU ml− 1 for 5 min) to the same patches, ethanol potentiated all wild-type bslo channels. (b) Ethanol responses of bslo T107V channels were identical before and after AP is applied to the same I/O membrane patches. In both a and b, the scatter graphs depict ratios of NPo values (× 100) obtained in the presence and absence of ethanol; each data point represents an individual patch or oocyte. Ethanol responses in a given patch before and after AP are connected by a dotted line. The dashed line indicates the point at which NPo is unchanged by alcohol.