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TLR2 and TLR4 protein expression was measured in freshly isolated and pooled human monocytes from healthy volunteers (n = 3 volunteers/experiment) after glucose challenge by flow cytometry as described in research design and methods. Values are expressed as MFI/105 cells ± SD. *P < 0.05 vs. mannitol or 5.5 mmol/l glucose; n = 4 experiments. □, TLR2; ▪, TLR4.
