TABLE 5.
Phenotypic characteristics of the potential superinfection exclusion systems using phage sk1, including the ratio of transcript levels of the newly identified Sie proteins under the control of the pNisA and p44 promoters of pNZ8048 and pNZ44, respectivelya
| Strain | EOP | Adsorption (%) | Cell death (%) | Transduction efficiency (%) | Ratio of transcript levels of pNisA to p44 | Predicted molecular mass (kDa) | pI | GC content (%) |
|---|---|---|---|---|---|---|---|---|
| NZ9000(pNZ44) | 1 | 100 | 100 | 100 | NTb | |||
| NZ9000(pNZ44sie2009) | ≤2.4 × 10−10 | 98.36 | 0 | 0.91 ± 0.27 | NT | 18.63 | 9.54 | 34.48 |
| NZ9000(pNZ44sie309) | 2.68 × 10−3 | 99.24 | 12.6 ± 6.37 | 5.99 ± 4.4 | 33.5 | 21.66 | 9.57 | 30.05 |
| NZ9000(pNZ44sie312) | 1.0 × 10−6 | 99.22 | 5.5 ± 2.39 | 0.47 ± 0.4 | 41.07 | 18.52 | 9.76 | 28.66 |
| NZ9000(pNZ44siemg2) | 7.5 × 10−6 | 99.16 | 5.26 ± 7.4 | 5.82 ± 0.36 | 30.9 | 18.62 | 9.74 | 27.24 |
| NZ9000(pNZ44sieT712) | ≤1.6 × 10−8 | 99.19 | 0 | 1.58 ± 0.27 | 140.6 | 22.35 | 9.58 | 33.33 |
| NZ9000(pNZ44orf2141mg3) | 1 | 99.22 | 99.99 ± 2.46 × 10−6 | 96.8 ± 2.3 | NT | 13.26 | 4.78 | 36.31 |
a
Predicted features of the Sie proteins including their predicted molecular mass, isoelectric point, and GC% content.
b
NT, not tested.