Figure 11. Structure and biosynthesis of the E. coli R1 core.
Organization of the waa locus is shown. Each gene has been mutated with a non-polar insertion, and the structures of LPSs from the resulting mutants were determined to generate the enzyme assignments shown with the structure (192, 228). Glycosyltransferases that form the inner core backbone are denoted by the yellow boxes and enzymes that modify the structure are in blue. Green boxes identify outer core glycosyltransferases and the ligase enzyme is in pink. The mobility of the mutant LPSs on a silver stained tricine-PAGE gel is also shown.