Figure 7. MSC cocultures do not prevent growth inhibitory effects of traditional chemotherapy.
A) HL60 cells (2.5 × 105 cells/ml) were cultured with MSC feeder layers (5 × 104 cells/ml) and treated with increasing concentrations of MX for 48 h. Apoptosis was quantitated by flow cytometric analysis of Annexin V staining. B) OCI-AML3 cells were cultured as in D) and the number of viable cells was quantitated by trypan blue exclusion;* = p<0.05, ** = p<0.01 from cells alone.
