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. Author manuscript; available in PMC: 2008 Oct 3.
Published in final edited form as: Nat Protoc. 2008;3(10):1537–1549. doi: 10.1038/nprot.2008.145

Table 2.

Cell, ASO, and Lipid Volumes for Transfection of Cultured Mammalian Cells

Volume fresh DMEM+10% FBS ASO mix Lipid mix
ASO (10 μM) DMEM Dharmafect 4 DMEM
24 well plate 450 μl / well 1.25 μl 23.75 μl 1 μl 24 μl
6 well plate 1.8 ml / well 5 μl 95 μl 4 μl 94 μl
10 cm Dish 9 ml / dish 25 μl 475 μl 20 μl 480 μl