Figure 4.
Effect of cadherin-reactive beads on the expression of various sarcomeric components in C2 cells. C2 myoblasts were plated overnight on gelatin-coated coverslips at 20% confluence, and beads coated with BSA or with the cadherin ligands were added. After additional incubation for 48 h after application of beads, cells were permeabilized, fixed, and labeled for the various muscle proteins. The number of positive cells in 10 randomly chosen microscopic fields (with 40× objective) was determined for each sample at every time point.